 |
NAME | METHOD | REFERENCE RANGE | UNITS |
 |
LAC Screen Result | CLOT DET | < 1.21 |
|
TYPE | VOLUME | TEMPERATURE | INSTRUCTIONS |
| PRIMARY | ||||
| 1 | Plasma Citrated | 2 (1) mL | Frozen - 14 Day(s) | See COLLECTION INSTRUCTIONS for platelet-poor plasma. |
| The presence of Lupus Anticoagulant can cause hypercoagulable states, as well as fetal loss. Due to its heterogeneous nature, no single assay can absolutely identify the presence of LA in every test plasma. Other LA tests should be performed if LAC Confirm is negative. |
|
Instructions for platelet-poor plasma:
1. Draw a plain red top tube to remove tissue fluid contamination. Discard this tube. 2. Draw blood into a buffered citrate collection tube (light blue top) filled to the proper level. Do not overfill. 3. The blood-to-anticoagulant ratio should be 9:1; inadequate filling of the collection device will decrease this ratio and may lead to inaccurate results. 4. Adjust the final citrate concentration in the blood of patients who have hematocrit values above 0.55 (55%). For hematocrits below 20%, there are no current data to support a recommendation for adjusting the citrate concentration. 5. The needle gauge could be between 19-22. For pediatric patients a 21-23 gauge needle may be used. 6. Invert gently 6 times to mix. Process immediately. 7. Centrifuge for 15 min at 2500 x g. 8. Remove plasma using a plastic pipette and transfer to a new tube. 9. Repeat centrifugation at 2500 x g for 15 min to assure complete platelet removal. 10. Dispense the plasma into 2 or more plastic tubes using a plastic transfer pipette. Label tubes appropriately. 11. Freeze immediately. Stable for 2 months at -20 C and 6 months at -70 C. 12. Specimen must remain frozen at all times. Ship to Specialty within 24 hours on dry ice. 13. Specimen should not be submitted if it is hemolyzed, microclots are present, the tube is less than 90% filled or a specimen with hematocrit >55% is collected without anticoagulant adjustment. |
