|Plasminogen||NEPH||6 - 25||mg/dL|
|1||Plasma Citrated||1 (0.5) mL||Frozen - 2 Month(s)||See COLLECTION INSTRUCTIONS for platelet-poor plasma.|
|Plasma ACD||1 (0.5) mL||Frozen - 2 Month(s)|
|Plasma EDTA||1 (0.5) mL||Frozen - 2 Month(s)|
|Plasminogen is the precursor of plasmin, and is converted to plasmin by tissue plasminogen activator (tPA) or urokinase-type plasminogen activator (uPA). The role of plasmin is to lyse fibrin clots and intact fibrinogen, and to inactivate factors Va and VIIIa. Plasminogen deficiency results in higher risk to venous thrombosis, and is also associated with ligneous conjunctivitis. Plasminogen levels may be monitored during thrombolytic therapy and in this case plasminogen levels decrease. Note, that plasminogen levels may increase during pregnancy, in acute phase reaction, in prostatic carcinoma and after vigorous exercise, and decrease in liver disease, disseminated intravascular coagulation, and with hereditary plasminogen deficiency. Newborn levels are about 60% of adult values, and reach adult values by age 6 months. Functional results are reported in % units, where 100% is the value expected in normal plasma. The reference range is approximately 75% to 130%. Antigen results are reported in mg/dL, however, it should be noted that antigen assays will not detect qualitative deficiencies (dysfunctional plasminogen).|
Instructions for platelet-poor plasma:
1. Draw a plain red top tube to remove tissue fluid contamination.
Discard this tube.
2. Draw blood into a buffered citrate collection tube (light blue
top) filled to the proper level. Sodium citrate of 0.109M should be
used. Use of other anticoagulants is unacceptable.
3. Invert gently 6 times to mix. Process immediately.
4. Centrifuge for 15 min at 2500 x g.
5. Remove plasma using a plastic pipette and transfer to a new tube.
6. Repeat the centrifugation at 2500 x g for 15 min to assure
complete platelet removal.
7. Dispense the plasma into 2 or more plastic tubes using a plastic
transfer pipette. Label tube appropriately.
8. Freeze immediately at -70 C.
9. Specimen must remain frozen at all times. Ship to Specialty within
24 hours on dry ice.
10. Specimen should not be submitted if it is hemolyzed,
microlots are present, the tube is less than 90% filled or specimen
with hematocrit >55 is collected without anticoagulant adjustment.
>10,000 platlets may cause false negative results.