 |
NAME | METHOD | REFERENCE RANGE | UNITS |
 |
Protein C Antigen | EIA | 65 - 140 | % |
|
TYPE | VOLUME | TEMPERATURE | INSTRUCTIONS |
| PRIMARY | ||||
| 1 | Plasma Citrated | 1 (0.5) mL | Frozen - 14 Day(s) | See COLLECTION INSTRUCTIONS for platelet-poor plasma. |
| Congenital Protein C deficiency is characterized by recurrent venous thrombosis and thromboembolism beginning in adolescence. Type I is characterized by reduction in both antigenic and functional levels, whereas in Type II functional levels are decreased much more than antigenic levels. |
|
Instructions for platelet-poor plasma:
1. Draw a plain red top tube to remove tissue fluid contamination. Discard this tube. 2. Draw blood into a buffered citrate collection tube (light blue top) filled to proper level. Do not overfill. 3. The blood-to-anticoagulant ratio should be 9:1; inadequate filling of the collection device will decrease this ratio and may lead to inaccurate results. 4. Adjust the final citrate concentration in the blood of patients who have hematocrit values above 0.55 (55%). For hematocrits below 20%, there is no current data to support a recommendation for adjusting the citrate concentration. 5. The needle gauge could be between 19-22. For pediatric patients a 21-23 gauge needle may be used. 6. Invert gently 6 times to mix. Process immediately. 7. Centrifuge for 15 min at 2500 x g. 8. Remove plasma using a plastic pipette to transfer into a new tube. 9. Repeat centrifugation at 2500 x g for 15 minutes to assure complete platelet removal. 10. Dispense the plasma into 2 or more plastic tubes using a plastic transfer pipette. Label tubes appropriately. 11. Freeze immediately at -70 C. 12. Specimen must remain frozen at all times. Ship to Specialty within 24 hours on dry ice. 13. Specimen should not be submitted if: * it is hemolyzed * microclots are present * the tube is less than 90% filled * a specimen with hematocrit >55% is collected without anticoagulant adjustment. |
