5990 Thrombotic Risk AssessR™

Fibrinogen CLOT DET  185 - 434  mg/dL 
Homocysteine UltraQuant ENZYMATIC  view ref range  umol/L 
Lipoprotein(a) view ref range  mg/dL 

Specimen Requirements
1 Plasma Citrated 2 (1) mL   Frozen - 14 Day(s) See COLLECTION INSTRUCTIONS for platelet-poor plasma. 
2 Serum 2 (1) mL   Ambient - 48 Hour(s), Refrigerated - 7 Day(s), Frozen - 2 Month(s)  

Clinical utility
Elevated levels of fibrinogen, Lipoprotein (a) and homocysteine are associated with increased risk of cardiovascular disease.

Collection Instruction
Instructions for platelet-poor plasma:
1. Draw a plain red top tube to remove tissue fluid contamination.
Discard this tube.
2. Draw blood into a buffered citrate collection tube (light blue
top) filled to the proper level.
3. The blood-to-anticoagulant ratio should be 9:1; inadequate
filling of the collection device will decrease this ratio and
may lead to inaccurate results.
4. Adjust the final citrate concentration in the blood
of patients who have hematocrit values above 0.55 (55%). For
hematocrits below 20%, there are no current data to
support a recommendation for adjusting the citrate concentration.
5. The needle gauge could be between 19-22. For pediatric
patients a 21-23 gauge needle may be used.
6. Invert gently 6 times to mix. Process immediately.
7. Centrifuge for 15 min at 2500 x g.
8. Remove plasma using a plastic pipette and transfer to a new tube.
9. Repeat centrifugation at 2500 x g for 15 min to assure
complete platelet removal.
10. Dispense the plasma into 2 or more plastic tubes using a plastic
transfer pipette. Label tubes appropriately.
11. Freeze immediately. Stable for 2 months at -20 C and 6 months at
-70 C.
12. Specimen must remain frozen at all times. Ship to Specialty within
24 hours on dry ice.
13. Specimen should not be submitted if it is hemolyzed, microclots
are present, the tube is less than 90% filled or a specimen with
hematocrit >55% is collected without anticoagulant adjustment.

For Homocysteine: Serum specimen must be centrifuged within one hour
of collection. Slightly higher values are observed in non-fasting

Reported: Same day

CPT Code: 83090, 83695, 85384