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NAME | METHOD | REFERENCE RANGE | UNITS |
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Specimen Source | |||
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BK (Polyomavirus) DNA Ultraquant | RT-PCR | < 200 | copies/mL |
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BK (Polyomavirus) DNA (log 10) | CALC | < 2.3 |
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TYPE | VOLUME | TEMPERATURE | INSTRUCTIONS |
PRIMARY | ||||
1 | Plasma EDTA | 2 (1) mL | Refrigerated - 7 Day(s), Frozen - 30 Day(s) | |
ALTERNATE | ||||
Plasma ACD | 2 (1) mL | Refrigerated - 7 Day(s), Frozen - 30 Day(s) | ||
Urine | Refrigerated - 7 Day(s), Frozen - 30 Day(s) | |||
Whole Blood ACD | 3 (1.5) mL | Ambient - 4 Day(s), Refrigerated - 4 Day(s) | ||
Whole Blood EDTA | 3 (1.5) mL | Ambient - 4 Day(s), Refrigerated - 4 Day(s) |
BK virus-associated nephropathy has become increasingly recognized as the cause of renal dysfunction and kidney allograft loss in transplant patients. The BK virus can reactivate and cause disease in states of immunodeficiency, most frequently in renal and bone marrow transplant patients. BK virus disease is associated with hemorrhagic and non-hemorrhagic cystitis, ureteral stenosis, tubulointerstitial nephritis, compromised allograft function, and allograft failure. The diagnosis of the BK virus has relied heavily on biopsy-based evidence. This new quantitative PCR-based assay can aid in the diagnostic and therapeutic management of BKV nephropathy. Recent studies demostrated that cidofovir may be useful in treating interstitial nephritis. Quantitative viral load testing may be useful for early diagnosis of reactivated BKV infection and for monitoring the response to specific therapy. |
Collect whole blood in EDTA K3 or ACD-A tube. Mix well. Centrifuge
and separate plasma. Place plasma in plastic tube for shipping. Ship on cold pack or frozen on dry ice (preferred) by overnight courier. |