8149 BK (Polyomavirus) DNA UltraQuant®

Components
NAME METHOD REFERENCE RANGE UNITS
Specimen Source      
BK (Polyomavirus) DNA Ultraquant RT-PCR  < 200  copies/mL 
BK (Polyomavirus) DNA (log 10) CALC  < 2.3   


Specimen Requirements
TYPE VOLUME TEMPERATURE INSTRUCTIONS
PRIMARY
1 Plasma EDTA 2 (1) mL   Refrigerated - 7 Day(s), Frozen - 30 Day(s)  
ALTERNATE
  Plasma ACD 2 (1) mL   Refrigerated - 7 Day(s), Frozen - 30 Day(s)  
  Urine   Refrigerated - 7 Day(s), Frozen - 30 Day(s)  
  Whole Blood ACD 3 (1.5) mL   Ambient - 4 Day(s), Refrigerated - 4 Day(s)  
  Whole Blood EDTA 3 (1.5) mL   Ambient - 4 Day(s), Refrigerated - 4 Day(s)  


Clinical utility
BK virus-associated nephropathy has become increasingly recognized as the cause of renal dysfunction and kidney allograft loss in transplant patients. The BK virus can reactivate and cause disease in states of immunodeficiency, most frequently in renal and bone marrow transplant patients. BK virus disease is associated with hemorrhagic and non-hemorrhagic cystitis, ureteral stenosis, tubulointerstitial nephritis, compromised allograft function, and allograft failure. The diagnosis of the BK virus has relied heavily on biopsy-based evidence. This new quantitative PCR-based assay can aid in the diagnostic and therapeutic management of BKV nephropathy. Recent studies demostrated that cidofovir may be useful in treating interstitial nephritis. Quantitative viral load testing may be useful for early diagnosis of reactivated BKV infection and for monitoring the response to specific therapy.


Collection Instruction
Collect whole blood in EDTA K3 or ACD-A tube. Mix well. Centrifuge
and separate plasma. Place plasma in plastic tube for shipping. Ship
on cold pack or frozen on dry ice (preferred) by overnight courier.


Reported: Same day

CPT Code: 87799

Notes: BK (Polyomavirus) DNA UltraQuant will quantify viral load within a defined range of less than 200 to more than 10,000,000,000 copies/mL. Extremely elevated viral loads are possible, especially in urine samples. This test is not approved for the testing of patient samples from New York State.